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Welcome!

The main focuses of our group is single cell systems biology.  We use super-resolution and live cell microscopy to study gene regulatory networks in cells and organisms. 

We demonstrated a technique that can multiplex detection of 32 mRNAs in single cells using  super-resolution barcoding [link].  We have recently showed that a sequential in-situ hybridization (SeqFISH) method can profile large number of RNAs in single cells [link]. We are working on expanding the throughput of the technique to over 100 genes and applying it to multi-cellular organisms to study developmental processes.

The Cai Lab, 11-21-2017 at Eaton Canyon

News & Events

06.07.2018
Our new paper published on Cell!
"Dynamics and Spatial Genomics of the Nascent Transcriptome by Intron seqFISH"

11.28.2017
Our new Nature Communications paper published!
"Identification of a neural crest stem cell niche by Spatial Genomic Analysis"

11.13.2017
Our new Nature Methods paper published!
"Profiling the transcriptome with RNA SPOTs"

07.05.2017
Our paper about MEMOIR is highligted on Caltech news!
"The Allen Discovery Center for Cell Lineage Tracing"

05.09.2017
Our new paper published on Biophysical Journal!
"Multiplexed Dynamic Imaging of Genomic Loci by Combined CRISPR Imaging and DNA Sequential FISH"

01.05.2017
Our recent paper about MEMOIR is highlighted by Nature News! "Systems biology: Molecular memoirs of a cellular family"

11.21.2016
Our new Nature paper published!
"Synthetic recording and in situ readout of lineage information in single cells"

10.19.2016
Demonstration of 200 mRNAs multiplexing in brain tissues!
"In Situ Transcription Profiling of Single Cells Reveals Spatial Organization of Cells in the Mouse Hippocampus"

Caltech    last update: 11/29/2017

 

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