The main focuses of our group is single cell systems biology.  We use super-resolution and live cell microscopy to study gene regulatory networks in cells and organisms. 

We demonstrated a technique that can multiplex detection of 32 mRNAs in single cells using  super-resolution barcoding [link].  We have recently showed that a sequential in-situ hybridization (SeqFISH) method can profile large number of RNAs in single cells [link]. We are working on expanding the throughput of the technique to over 100 genes and applying it to multi-cellular organisms to study developmental processes.

The Cai Lab, 11-21-2017 at Eaton Canyon

News & Events

Our new paper published in Nature!
"Transcriptome-scale super-resolved imaging in tissues by RNA seqFISH+"

Our new paper published in Cell!
"Dynamics and Spatial Genomics of the Nascent Transcriptome by Intron seqFISH"

Our new Nature Communications paper published!
"Identification of a neural crest stem cell niche by Spatial Genomic Analysis"

Our new Nature Methods paper published!
"Profiling the transcriptome with RNA SPOTs"

Our paper about MEMOIR is highligted on Caltech news!
"The Allen Discovery Center for Cell Lineage Tracing"

Our new paper published on Biophysical Journal!
"Multiplexed Dynamic Imaging of Genomic Loci by Combined CRISPR Imaging and DNA Sequential FISH"

Our recent paper about MEMOIR is highlighted by Nature News! "Systems biology: Molecular memoirs of a cellular family"

Our new Nature paper published!
"Synthetic recording and in situ readout of lineage information in single cells"

Demonstration of 200 mRNAs multiplexing in brain tissues!
"In Situ Transcription Profiling of Single Cells Reveals Spatial Organization of Cells in the Mouse Hippocampus"

Caltech    last update: 11/29/2017